Histology and Fluorescence Microscopy

The histology or histopathology is the study of microscopic anatomy of cells, tissues, diseased tissue or cancer. It is performed by examining the sample under a light microscope. The ability to visualize or differentially identify microscopic structures is the main challenge for the Visitron HistoScope Imaging System. To get the best resolution and true colour information for the correct analysis and diagnosis we use state of the art colour CCD cameras with three different technologies. Colour mosaic CCD, Pixel shifting CCD technique and liquid crystal three shot sequential RGB channel acquisition. The easy to use VisiView imaging software, developed by Visitron Systems GmbH controls the camera acquisition, colour correction, overlay and analysis of the images. In addition motorized microscopes are also supported e.g. for screening of cells or tissue.

The majority of fluorescence microscopes, especially those used in the life sciences, are of the epifluorescence design. Light of the excitation wavelength is focused on the specimen through the objective lens. The fluorescence emitted by the specimen is focused to the detector by the same objective that is used for the excitation which for greater resolution will need objective lens with higher numerical aperture. Since most of the excitation light is transmitted through the specimen, only reflected excitatory light reaches the objective together with the emitted light and the epifluorescence method therefore gives a high signal-to-noise ratio. An additional wavelength specific filter between the objective and the detector can filter out the remaining excitation light from fluorescent light. Visi4 provides high effiency filter sets and intelligent microscope illumination systems which match you applications and fits to your imaging system.

Visi4 offers fiber coupled  HXP/XCite - DG4/5 - High Speed Polychromatic Illumination - CoolLed.